Part:BBa_K2680522:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

iGEM 2019 Characterization

As we were interested in utilizing quorum sensing as a vital component of our project, we characterized BBa_K2680522 with our circuit BBa_K3059769. In order to test out how sensitive our receiver circuit is to LuxI HSL production, we decided to measure Fluorescence over time in a plate reader experiment after induction with varying concentrations of HSL, ranging from 1nM to 10uM.

In the graph below, each data point represents the average fluorescence / OD600 value of 5 individual datapoints. The error bars are +/- 2 standard errors. The y-axis uses a Log10 scale as fluorescence is being measured.

As can be seen in the graph, BBa_K2680522 has a very steep induction curve occurring between 10nM and 100nM. This is useful for us, and other iGEM teams to know, as this switch like behavior is useful for distance-dependent patterning.

User Reviews

UNIQ32179a965a4c6eb3-partinfo-00000001-QINU UNIQ32179a965a4c6eb3-partinfo-00000002-QINU